Coding
Part:BBa_K1321114:Design
Designed by: Xenia Spencer-Milnes Group: iGEM14_Imperial (2014-10-09)
CBDclos fused to phytochelatin (PC) EC20 with LacI promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 230
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. The LacI vector was fused to the protein fusion using the Xba and SpeI site.
Source
Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111. The LacI part came from BBa_J04500.